Biological Markers to Study the Morphological Modifications Induced by Perinatal Asphyxia

KÖLLIKER-FRERS RODOLFO; JUAN P LUACES; MARIA INÉS HERRERA; MARTINEZ MICAELA; SOFIA BORDET; CHEVALIER GUENSON; CARLOS F KUSNIER; CAO, GABRIEL; LUCAS UDOVIN; NICOLAS TORO URREGO; TAMARA KOBIEC; GRACIELA OTTAVIANO; SANTIAGO PEREZ-LLORET; LOSADA, MATILDE OTERO; FRANCISCO CAPANI

Congreso; XVI Congreso Interamericano de Microscopía (CIASEM). Ciudad de Oaxaca, México, del 25 al 28 de octubre de 2022; 2022

For this study, we used a well-established murine model of PA [1]. After one, 2, 4 and 6 months of severe PA (21 min) rats were sacrificed and their brains were analyzed by combining photooxidation, conventional electron microscopy and electron tomography 3-D reconstruction techniques in two areas hypoxia sensible: neostriatum and hippocampus [1]. Findings: After one month of PA, we found an increase in the F-actin staining in neostriatal and hippocampal dendritic spines together with some filopodia-likes structures, a typical embryonic type of spines in photooxidated tissue [2] [Fig 1 A). In contrast, after second month of PA, spines were less consistent stained. In addition, we observed an increment of marker for neuronal and glial dysfunction such as GFAP, neurofilament and MAP-2 [3]. These modifications were more clearly defined after 4 months of PA [3]. After 6 months of PA postsynaptic densities (PSDs) in neostriatum were highly modified. Using three-D reconstructions and electron tomography we were able to find clear signs of degeneration in the asphyctic PSDs (Fig 1 B and C) [3]