Localization of thioredoxin family proteins in the rat central nervous system. Immunocytochemical study

CAPANI, FRANCISCO; AON-BERTOLINO, MARÍA LAURA; ROMERO, JUAN; GALEANO, PABLO; HOLUBIEC, MARIANA; BADORREY, MARÍA SOL; SARACENO, GUSTAVO EZEQUIEL; LILLIG, CHRISTOPHER HORST.

Corea del Sur, Busan

Congreso; Congreso.22nd Biennial Meeting of the International Society of Neurochemistry and Asian-Pacific Society for Neurochemistry. 2009; 2009

Glutamate excitotoxicity, oxidative stress and mitochondrial dysfunction have been proposed as one of main causes of neuronal damage and glial reaction induced by a hypoxic ischemic episode. Although most animal model of hypoxia-ischemia use rats to study the mechanisms involved in the neuronal cell death during different kinds of hypoxia-ischemia episodes, there is not a complete and clear statement of the localization of the thiol protein in the central nervous system (CNS). The main aim of this work is to study the distribution of the following thioredoxin proteins family in rat CNS: mTrx-1, hTrx-2, TRrx-2, TRrx-1 Txnip, Grx-1, Grx-2, Grx-3, Grx-5, and gGCS, Prx-1, Prx-2, Prx-3, Prx-4, Prx-5 and Prx-6. In this study we have analyzed the most sensitive area to hypoxia-ischemic insult of the CNS including: cerebellum, neostriatum, hippocampus, spinal cord, substantia nigra, cortex and retina. Although previous studies have suggested that these proteins are distributed in most of the cell types and regions of the CNS, we have observed several differences in their intensity and regional distribution. According to our knowledge this study is the first effort for describing, the precise localization of the thioredoxin proteins family in the CNS of the rat. We think that these data might help to reveal new insights about the role of the oxidative pathway in the pathogenesis of the brain hypoxia-ischemia. Supported by UBACYT M407, PID 5784. DFGSFB593-N01, D0805.