Effect of alkanols on cell growth, sporulation, and lipase production of Bacillus licheniformis S-86

TORRES SEBASTIÁN; BAIGORÍ MARIO; CASTRO GUILLERMO R.

Boston, MA, USA

Congreso; 224th ACS (American Chemical Society) National Meeting.; 2002

American Chemical Society

(Abstract 44). Alcohol-tolerant Bacillus licheniformis S-86 produces stable lipases in organic solvents. The effect of seven alkanols on cell growth, sporulation and lipase production was studied. Relationship between maximal alcohol concentration tolerated and logP were for 2,3-butanediol 6.0%:-0.86; ethanol 4.0%:-0.24; propan-2-ol 3.0%:0.28; butan-1-ol 0.9%:0.80; 3-ethylbutan-1-ol 0.6%:1.30; hexan-1-ol 0.1%:1.86; and heptan-1-ol 0.05%:2.39, respectively. Alkanols addition reduced biomass after exponential growth phase between 30%(0.4% 3-methylbutan-1-ol) to about 90%(0.1% hexan-1-ol). Specific activity of lipases was approximately two folds higher in cultures supplemented with C3 to C5 alkanols than the control. Two different lipases activities were observed. One of them was inhibited by PMSF and the other did not. In presence of 0.4% 3-methylbutan-1-ol, PMSF-sensitive and PMSF-resistant lipase specific activities were increased 2 times and 4 times, respectively. Sporulation was strongly depleted compared to the control by 104 folds CFU/ml in presence of 3-methylbutan-1-ol. Lipases production could not be linked to the sporulation process.