Organic solvent resistant lipase produced by thermoresistant bacteria

TORRES SEBASTIÁN; CASTRO GUILLERMO R.

New Horizons in Biotechnology

Kluwer Academic Publishers

Lugar: Netherlands; Año: 2003; p. 113 - 122

A thermoresistant microbial wild-type strain designed S-86, which can be grown in presence of 3-methylbutanol in the medium, and can produce lipolytic enzyme was isolated. The S-86 strain was identified as a member of the Bacillus genus and belonged to the subtilis group. It is tentatively named B. licheniformis. Exocellular lipase was detected during stationary growth phase, and displayed high enzymatic activity in 50 % homogenous solvent mixtures of glycerol, ethylenglycol, propilenglycol, and methanol. Highest lipase activity was detected in 25 to 50 % glycerol-water mixtures, and optimal reaction temperature in the range of 65 to 70°C in both solvent systems.