Secretomes from dysfunctional perivascular adipose tissue upregulate nadph oxidase and adipokines in vascular smooth muscle cells

CEJAS, J; QUESADA, I; CASTRO C

MENDOZA

Congreso; XXXII Reunión Científica Anual De La Sociedad De Biología De Cuyo; 2016

Sociedad de Biologia de Cuyo

Perivascular adipose tissue (PVAT) is able to release molecules with varied paracrine effects on the underlying vascular cells. Type 2 diabetes can affect PVAT causing abnormal fat cells and infiltration of inflammatory cells producing an imbalance between PVAT derived factors. Analysis of PVAT secretome allows to study the paracrine effect of these molecules on vascular smooth muscle cells (VSMC) and its contribution to atherogenesis. Previous studies from our group showed that PVAT secretome of fructose fed ApoE-KO mice significantly increases the generation of reactive oxygen species (ROS). The aim of this study was to analyze the expression of the primary ROS-generating enzyme in vascular cells, NADPH oxidase and the proinflammatory adipokines visfatin and resistin in VSMC treated with secretomes obtained from PVAT of C57BL / 6 mice (WT-s), ApoE- deficient mice (ApoE-KO-s) and fructose fed ApoE-KO mice (ApoE-KO / Fs). Secretomes were obtained from 400 mg of periaortic adipose tissue in 1 ml of DMEM / F-12 at 37 ° C for 4 hours. Gene expression levels were determined by qRT-PCR. We first found that treatment with WT-s induce no significant gene expression changes compared to untreated cells. However, ApoE-KO-s significantly increased (p