Quercetin inhibits angiotensin II-induced aortic smooth muscle cell migration

GIL LORENZO ANDREA; CRUZADO, MONTSERRAT; CASTRO, CLAUDIA

Mendoza

Congreso; XXVI Reunión de la Sociedad de Biología de Cuyo; 2008

Sociedad de Biología de Cuyo

QUERCETIN INHIBITS ANGIOTENSIN II-INDUCED AORTIC SMOOTH MUSCLE CELL MIGRATION Gil Lorenzo AF?, Castro CM#*, Cruzado MC# . ?Program I+D SeCTyP, #FCM, UNCuyo, *IMBECU, CONICET. Smooth muscle cell (SMC) migration into the vascular wall is an important process of vascular remodeling associated with hypertension. Quercetin, a bioflavonoid present in the red wine is known to have antioxidant properties and a protective role in cardiovascular system. In this study we investigated the effect of quercetin (5 and 10 μg/mL) on cultured aortic SMC from spontaneously hypertensive rats (SHR). We evaluated angiotensin II(AII)-stimulated migration by scrape-wound assay and cell viability by CellTiter assay. Data (mean±SEM) presented as percent of 0.1%fetal calf serum used as control, were analyzed by ANOVA and post test of Bonferroni. AII (10-7M) induced a significant increase of SMC migration after 24 h stimulation (198.23±10.67). The highest concentration of quercetin markedly inhibited AII-stimulated cell migration (129.29±9.88, *P