INVESTIGADORES
CALVO Ernesto Julio
artículos
Título:
Layer-by-Layer Self-Assembled Osmium Polymer-Mediated Laccase Oxygen Cathodes for Biofuel Cells: The Role of Hydrogen Peroxide
Autor/es:
PABLO SCODELLER; ROMINA CARBALLO; RAFAEL SZAMOCKI; LAURA LEVIN; FLAVIA FORCHIASSIN; ERNESTO J. CALVO
Revista:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Columbus; Año: 2010 vol. 132 p. 1132 - 1140
ISSN:
0002-7863
Resumen:
High potential purified Trametes trogii laccase has been studied as a biocatalyst for oxygen
cathodes composed of layer-by-layer self-assembled thin films by sequential immersion of mercaptopropane
sulfonate-modified Au electrode surfaces in solutions containing laccase and osmium-complex bound to
poly(allylamine), (PAH-Os). The polycation backbone carries the Os redox relay, and the polyanion is the
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
cathodes composed of layer-by-layer self-assembled thin films by sequential immersion of mercaptopropane
sulfonate-modified Au electrode surfaces in solutions containing laccase and osmium-complex bound to
poly(allylamine), (PAH-Os). The polycation backbone carries the Os redox relay, and the polyanion is the
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
Trametes trogii laccase has been studied as a biocatalyst for oxygen
cathodes composed of layer-by-layer self-assembled thin films by sequential immersion of mercaptopropane
sulfonate-modified Au electrode surfaces in solutions containing laccase and osmium-complex bound to
poly(allylamine), (PAH-Os). The polycation backbone carries the Os redox relay, and the polyanion is the
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O2
-Os). The polycation backbone carries the Os redox relay, and the polyanion is the
enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. Enzyme
thin films were characterized by quartz crystal microbalance, ellipsometry, cyclic voltammetry, and oxygen
reduction electrocatalysis under variable oxygen partial pressures with a rotating disk electrode. New kinetic
evidence relevant to biofuel cells is presented on the detection of traces of H2O2, intermediate in the O22O2, intermediate in the O2
reduction, with scanning electrochemical microscopy (SECM). Furthermore the inhibitory effect of peroxide
on the biocatalytic current resulted in abnormal current dependence on the O2 partial pressure and peak
shape with hysteresis in the polarization curves under stagnant conditions, which is offset upon stirring
with the RDE. The new kinetic evidence reported in the present work is very relevant for the operation of
biofuel cells under stagnant conditions of O2 mass transport.
shape with hysteresis in the polarization curves under stagnant conditions, which is offset upon stirring
with the RDE. The new kinetic evidence reported in the present work is very relevant for the operation of
biofuel cells under stagnant conditions of O2 mass transport.
2 partial pressure and peak
shape with hysteresis in the polarization curves under stagnant conditions, which is offset upon stirring
with the RDE. The new kinetic evidence reported in the present work is very relevant for the operation of
biofuel cells under stagnant conditions of O2 mass transport.2 mass transport.