Molecular wiring enzymes in organized nanostructures

ERNESTO JULIO CALVO; C. DANILOWICZ,; A. WOLOSIUK,

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Año: 2002 p. 2452 - 2453

0002-7863

We report on the “molecular wiring” efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have investigated the mechanisms of electrical signal generation from biomolecular recognition. The specific rate of bimolecular FADH2 oxidation (“wiring efficiency”) is limited by the diffusionlike electron hopping mechanism in the multilayers.molecular wiring” efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have investigated the mechanisms of electrical signal generation from biomolecular recognition. The specific rate of bimolecular FADH2 oxidation (“wiring efficiency”) is limited by the diffusionlike electron hopping mechanism in the multilayers.2 oxidation (“wiring efficiency”) is limited by the diffusionlike electron hopping mechanism in the multilayers.