Biotherapeutic potencial of a biosurfactant extract obtained from Pseudomonas syringae pv tabaci

GEORGINA MARTINI; BIBIANA B. NERLI; SOLEDAD BUSTILLO; LUCIANA PELLEGRINI MALPIEDI

Simposio; Simposio Latinoamericano De Tecnología De Cultivos Celulares (IX SLATCC); 2022

The interest in biosurfactants (BS) has increased in recent years due to their biodegradability, biological properties and the possibility of production by fermentation. However, there are few publications on their potential antitumor activity. Thus, in this work we evaluate the effect of a lipopeptides (LP) extract produced by P. syringae pv. tabaci on tumoral cell adhesion and migration. Firstly, cytotoxicity of LP (0.625-10 mg/mL) was assessed in a murine tumoral cell line, LM3 (CVCL_D269), grown in DMEM-5%FBS at 37°C-5%-CO2 for 3-24h. Non-cytotoxic concentrations were selected for both assays. For cellular adhesion evaluation, cells (3×104/well) were preincubated for 30 min at 37°C with LP (0.625-5 mg/mL) or culture medium (control). Adherent cells were fixed and stained with crystal violet and the percentage of cell adhesion was determined. Cell migration was measured by wound-healing assay. Cells were grown in a 6-well plate and a wound was created by a sterile pipette tip. Culture medium alone or LP (2 mg/mL) were added to the cells and incubated for 24 h. Wound widths were measured and percentage of cell migration was calculated. Results showed that the lowest dose assayed (0.625 mg/mL) evidenced a 17.7% of adhesion inhibition effect and with 5 mg/mL, 58.5% of cells didn´t adhere to the substrate. Moreover, the control wound scratch almost closed after 24 h but treatment with 2 mg/mL of LP resulted in the suppression of cell migration in a 37.3 %. Although more studies are needed, these findings demonstrate the therapeutic potential of these biosurfactants.