DEVELOPMENTAL TOXICITY OF BISPHENOL A DIGLYCIDYL ETHER (EPOXIDE RESIN BADGE) DURING THE EARLY LIFE CYCLE OF A NATIVE AMPHIBIAN SPECIES

IANINA HUTLER WOLKOWICZ HUTLER; GABRIELA SVARTZ; CAROLINA ARONZON; CRISTINA PÉREZ-COLL

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY

SETAC PRESS

Lugar: Buenos Aires; Año: 2016 vol. 35 p. 3031 - 3038

0730-7268

Bisphenol A, diglycidyl ether (BADGE) is used in packaging materials, epoxy adhesives, additive for plastics but is also a potential industrial wastewater contaminant. The aim of this study was to evaluate the adverse effects of BADGE on Rhinella arenarum, by means of standardized bioassays at embryo-larval development. The results showed that BADGE was more toxic to embryos than to larvae at all exposure times. At acute exposure, lethality rates of embryos exposed to concentrations from 0.0005 mg/L BADGE were significantly higher than vehicle control, whereas lethality rates of larvae were significantly higher from 10 mg/L BADGE. Then, the toxicity increased significantly, with a LC50-96 h of 0.13 and 6.9 mg/L BADGE for embryos and larvae respectively. By the end of the chronic period the LC50-336 h were 0.04 and 2.2 mg/L BADGE for embryos and larvae respectively. This differential sensitivity was also ascertained by the 24-h pulse exposure experiments, in which embryos showed a stage-dependent toxicity, being blastula the most sensitive stage and S.23 the most resistant one. The most important sublethal effects in embryos were cell dissociation and delayed development, whereas the main abnormalities observed in larvae were related to neurotoxicity, as scare response to stimuli and narcotic effect.