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VALDEZ susana Ruth

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Título:

HYPOPHYSITIS IN NON-OBESE DIABETIC MICE AS A NEW TISSUE- SPECIFIC AUTIMMUNE RESPONSE

Autor/es:

MORENO-SOSA MARÍA TAMARA; SOAJE MARTA; SÁNCHEZ MARÍA BELÉN; VALDEZ SUSANA R; YÚDICA SEDANA FLORENCIA; NEIRA FLAVIA J; PENNACCHIO GISELA E; PIETROBON ELISA O; JAHN GRACIELA A; MACKERN OBERTI, JUAN PABLO

Lugar:

Buenos Aires

Reunión:

Congreso; LXII Reunión Anual de la Sociedad de Investigación Clínica; 2017

Institución organizadora:

SAIC, SAIB, SAI, SAFE, SAA, SAB, SAFIS, SAA, SAP.

Resumen:

Type 1 diabetes mellitus is achronic organ-specific autoinmune disease that results from the destruction ofbeta (β) cells in the pancreatic islets and shows a pronounced leukocyteinfiltration in the gland. Non-obese diabetic (NOD) mice develop autoimmunediabetes approximately at 6 months of age in females, providing an experimentalmodel for human Type 1 Diabetes. They also displayadditional autoreactive immuneresponses against other glands includingthe prostate and thyroid.Furthermore, female NOD mice exhibit a lower fertility index compared to wildtype mice, that develops approximately at the same time as diabetes. Todetermine whether the low fertility of NOD mice is caused by an autoinmune responseagainst the hypophysis, we assessed the presence of leukocyte infiltration inthe gland. To this end, the presence of CD45+ (panleukocyte) cells in thehypophysis of 3 and 6 months old female BALB/c (control group) and NOD mice wasanalyzed by flow cycytometry.An increase in leukocyteinfiltration in the hypophysis from 6 months old NOD mice was observed comparedto the controls (0.05±0.04 % BALB/c mice vs 3.2±1.0% NOD mice; p<0.05),while young female NOD mice did not show infiltration. Furthermore, we foundthat the older female NOD mice displayed autoreactive IgGs against hypophysealtotal proteins measured by ELISA, as well asprolactin specific IgG, that werenot present in control mice. These results suggest that NOD mice may have anautoreactive immune response against the hypophysis that may lead to a decreasein the production of hormones such as FSH, LH and prolactin, resulting in adecrease in fertility. To our knowledge, this is the first report that demonstratesthe presence of hypophysitis in NOD mice. These resultsmay help understand theheterogeneity of symptoms present in autoimmune diseases.

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