EFFECTS OF CADMIUM EXPOSURE IN CEREBELLUM ? EFFECT OF DIETS AND AGE

ÁLVAREZ SILVINA M; PIGUILLEM, SILVANA N; VALDEZ, S; BIAGGIO VS; GIMENEZ MARÍA SOFÍA

San L

Congreso; Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

<!-- /* Font Definitions */@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Cambria;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}@page Section1{size:595.0pt 842.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:35.4pt;mso-footer-margin:35.4pt;mso-paper-source:0;}div.Section1{page:Section1;}-->Cadmium (Cd) is a toxic metal and an important environmentalcontaminant. We studied its effects on the oxidative stress markers and thehistoarchitecture of rat cerebelum under different diets. 4 lots of femaleWistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) asprotein source. Within each group, 1 lot received regular water (control-Co)and the other, 15 ppm of Cd in the drinking water for 60 days. On the otherhand, we also measured some oxidative markers in rat fetus´s cerebellum whosemother received Cd during the pregnancy.Cerebellums were isolated and total RNA was obtained withTrizol. Nrf 2, NOX and SOD were determined by RTPCR. S28 was used as thecontrol. Histological studies were performed in fixed Hematoxilin-Eosin-stainedtissuesIn adult rats, nrf2 and SOD decreased in Cd groups (p<0,05) but SODalso showed an increase in Soy-Cd. NOX decreased in Cas-Cd with no differencesin Cas groups. The histological studies showed that Cd groups have certain disorganization,especially in the Purkinje cells layer. When we compared the markers with thosefound in fetuses,we observed that NOX increased significantly in both Cdgroups (p>0.05) and that SOD increased only in Soy-Cd (p>0.05). Theseresults suggest that in the fetuses the response to oxidative stress induced byCd is better than the one found in adult rats, where the antioxidant system isdepressed. Further studies are necessary in order to learn the effect of Cd onthe fetuses? cerebellum oxidative stress metabolism and architecture.