Genetic diversity among isolates of Phaeoisariopsis griseola by means of ISSR markers

STENGLEIN S.A., FERMOSELLE G.E & BALATTI P.A.

Posadas, Misiones

Congreso; XL Congreso de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular; 2004

Phaeoisariopsis griseola (PG) is a fungus that causes considerable reductions of bean (Phaseolus vulgaris L.) yields. This pathogen, like other ones, coevolved with the host therefore fungal isolates have been clustered as Mesoamerican and Andean.  The total number of bands amplified was 93, being 68% of them polymorphic. Multivariate analysis was used to generate a fenogram that clustered isolates in Andean and Mesoamerican and based on the place of isolation. Statistical analysis resulted in the identification of 17 haplotypes of the fungus, that belong to both Mesoamerican and Andean type. Considering that the fungus has asexual reproduction diversity among them is considerable and related with the place of origin of the materials suggesting that the places are rather unconnected in terms of fungal spore dispersal. Finally, ISSR proved to be reliable tools not only to identify but also to cluster PG isolates and to detect diversity within isolates