Development of SRAP markers to evaluate the genetic variability in Brachypodium distachyon complex

DINOLFO M.; CASTAÑARES E.; MARTINEZ M.; ARROYO F.; STENGLEIN S.

BOTANY LETTERS

Taylor & Francis

Año: 2023 vol. 170 p. 644 - 651

Brachypodium distachyon has been accepted as a model grass species for genetics and molecular genomics in cereals since 2001. However, the genetic variability present in Brachypodium spp. continue being the aim of many research. Therefore, the aim of this work was to analyze the genetic variability present in different Brachypodium spp. from different countries and to find different ORFs among them by using SRAP markers. In our study, a total of 12 Brachypodium spp. accessions were selected from different countries. By using sequence-related amplified polymorphism (SRAP) markers, the genetic variability was evaluated and some fragments were sequenced to obtain differential open reading frames information among genotypes. SRAP molecular markers revealed high variability among accessions being USA the accession that showed most variability at the DNA level. The analysis of sequenced SRAP fragments from monomorphic and polymorphic fragments as well as those obtained from new primer combinations targeted coding regions into B. distachyon available genome. SRAP molecular marker showed not only to be an excellent tool for Brachypodium variability studies but also to provide additional information about differential amplification of open reading frames.