Phosphorylation of intracellular tyrosines modulates the ionotropic function of the alpha7 nicotinic receptor

CHRESTIA, F.; BOUZAT, C.; ESANDI, M.C.

Córdoba

Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2018

Sociedad Argentina de Investigación en Neurociencias (SAN)

Alpha7 is expressed in the brain and contributes to cognition, attention, and memory. It contains an extracellular domain with the agonist binding sites; a transmembrane domain, which forms the ion pore; and an intracellular domain (ICD), which contains sites for modulation and intracellular signaling. The mechanisms by which the cell can regulate the ionotropic function of alpha7 remain unknown. We explored how intracellular phosphorylation affects alpha7 activity by patch clamp recordings in HEK cells expressing alpha7. Wild-type alpha7 channel activity elicited by ACh appears as brief isolated openings and as activation episodes containing a few brief openings in quick succession (bursts). Preincubation of cells expressing α7 with the inhibitor of Src family kinases (PP2) increased significantly the mean burst duration. The exposure of cells to PP2 during the course of the recording revealed a significant increase in the frequency of channel opening in addition to the increase of burst durations. To confirm that these changes were due to the inhibition of phosphorylation of alpha7-ICD, we introduced mutations at potential phosphorylation sites (Y386F and Y442F). The mutations prolonged burst durations, thus mimicking the effects of PP2. Also, the mutants were insensitive to PP2, confirming that Y386 and Y442 are responsible for its effects on alpha7 kinetics. Our results indicate that dephosphorylation positively modulates alpha7 channel activity in a way compatible with decreased desensitization.