Fosfomycin penetration into swine leukocytes

DENISA S. PÉREZ GAUDIO1 GUADALUPE MARTÍNEZ, MARÍA B. FERNÁNDEZ PAGGI1,2,3, JULIETA M. DECUNDO, AGUSTINA ROMANELLI, SUSANA DIEGUEZ, ALEJANDRO L. SORACI,; DEN GUADALUPE MARTÍNEZ, MARÍA B. FERNÁNDEZ PAGGI1,2,3, JULIETA M. DECUNDO, AGUSTINA ROMANELLI, SUSANA DIEGUEZ, ALEJANDRO L. SORACI1,; DIÉGUEZ, SUSANA;; ALEJANDRO L. SORACI,

INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE

VETERINARY SOLUTIONS LLC

Año: 2019 vol. 16 p. 156 - 162

1559-4602

Fosfomycin (FOS) is an antibiotic widelyused in Asia and Latin-America for thetreatment and prevention of swine infections caused by resistant bacteria. Intracellular and interstitial fluids are biophases forfacultative intracellular microorganisms. Onthe other hand, the choice of an antibioticdepends on its direct antimicrobial activity,which is in vitro determined by the MIC.FOS MIC90 ranges from 0.25 to 0.50 μg/mL for the most important pathogens in pigproduction. The aim of this research was to study the in vitro and in vivo FOS penetration into swine leukocytes (white bloodcells-WBC) and to determine if intracellularconcentrations exceed the MIC90 for mostswine pathogens. For the in vivo, studysix weaning piglets were intramuscularlyadministered a 15 mg/kg dose of disodiumFOS. For the in vitro study, WBC obtainedfrom weaning piglets were incubated with a150 μg/mL dose of disodium FOS. Intracellular concentrations were analyzed by HPLCMS/MS and they ranged from 0.89 to 3.88μg/mL for the in vitro assay (Tmax: 4 h)and between 0.16 and 1.33 μg/mL for the invivo study (Tmax: 0.5 h). The values foundin both studies are above the MIC90 for the most important pathogens in swine production. These values were maintained for morethan 24 h for the in vitro study (T> MIC=24h) and for more than 4 h for the in vivostudy (T> MIC=4 h).