Determination of Antibiotic Fosfomicyn In Chicken Serum by Liquid Chromatography-Tandem Mass Spectrometry.

DIEGUEZ, S.; ALEJANDRO SORACI; TAPIA ; CARCIOCHI; PEREZ; HARKES; ROMANO,O.

JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES

Taylor & Francis,

Año: 2011 p. 116 - 128

1082-6076

Abstract A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of fosfomycin in chicken serum using fudosteine as the internal standard. Serum samples were treated with methanol to precipitate proteins. A subsequent clean up using liquid-liquid extraction followed by dilution was performed to eliminate phospholipids which are prone to produce unwanted matrix effects. The HPLC-MS/MS system involved the use of an isocratic mobile phase on a cyano stationary phase column and electrospray ion source operating in negative ion mode. Single reaction monitoring of transitions m/z 137 ?¨79 and 178 ?¨91 was performed on a triple quadrupole mass spectrometer to quantify fosfomycin and fudosteine, respectively. Response was linear over the range of 0.1 to 50 ?Êg mL−1. Recovery ranged from 95 to 108%. Accuracy determined for spiked samples at 5, 10, and 20 ?Êg mL−1 was −7.8, 1 and −0.7%, respectively, expressed as relative error. Within day and between days precision, in terms of coefficient of variation, were less than 10% for all concentrations. The developed method was successfully used in a pharmacokinetic study after oral administration of calcium fosfomycin to broiler chickens.