Vitro Penetration of Fosfomycin in Respiratory Cells

D.S. PÉREZ, A.L. SORACI, M.O. TAPIA

The pigs journal

Pigs journal

Lugar: Londres; Año: 2012 vol. 67 p. 1 - 25

1352-9749

bstract 26 27 Infectious diseases of the respiratory system are important in intensive swine production. 28 Fosfomycin is a widely used antibiotic for the treatment of swine lung infections. The aim of this 29 research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 30 cells). Cells cultures were subjected to four treatments: a) negative control; b) disodium fosfomycin 31 (280 µg/mL); c) disodium fosfomycin (130 µg/mL) and d) calcium fosfomycin (130 µg/mL). 32 Intracellular concentrations of fosfomycin were analyzed by HPLC MS/MS. Concentrations in HEp- 33 2 cells incubated with 280 µg/mL of disodium fosfomycin ranged from 0.74 to 2.79 µg/mL (Tmax: 12 34 h). When incubated with the same formulation of fosfomycin at a concentration of 130 µg/mL, 35 intracellular concentrations ranged between 0.31 and 1.60 µg/mL (Tmax: 12 h). Calcium fosfomycin 36 reached intracellular concentrations that varied between 0.46 and 1.11 µg/mL (Tmax: 8 h). 37 Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine 38 respiratory infections (Streptococcus sp.; 0.25 µg/mL). Therefore, it is apparent that fosfomycin is an 39 alternative for the treatment of intracellular respiratory infections in pigs.