Isolation and characterization of plant growth-promoting Pseudomonas from rhizozphere of maize

CORDERO, P.; FISCHER, S.; CAVIGLIASSO, A.; BUENO, M.; MORI, G.

Mar del Plata

Congreso; XLIII Reunión Anual de SAIB; 2007

SAIB

The aim of present study was to isolate PGPR spp. from grown in field of Cordoba region. Bacterial strains were isolated from bulk soil and rhizosphere and endorhizosphere of maize plants at different growth stages (flowering plants, in December, and physiological maturity, in March). Gram stain, biochemical tests and genetic analyses were performed. Isolates belonging to genus were screened by their ability to solubilize phosphate, to produce siderophores, indole acetic acid (IAA), extracellular enzymes and to inhibit phytopathogens fungi. Approximately, 240 isolates were obtained from maize root and soil in King B and Gould´s S1 medium. Gram negative, oxidase and catalase positive, motile and producer of pigment strains were confirmed by PCR as belonging to fluorescent group. The highest percent of PGPR characteristic was observed among populations from March. On the other hands, of strains from December, 39% were able to solubilize phosphate, 78%, 22% and 67% produce proteases, cellulases and siderophores respectively. Some isolates were able to produce IAA and others could inhibit the growth of . Conclusion: we obtained several spp. from maize root with PGPR trait in vitro. In the future, promotion of plant growth by these strains should be analyzed in greenhouse conditions