Determination of Ochratoxin A in beer samples by a novel and practical SALLE-based approach prior to UHPLC-MS/MS

LEONARDO MARIÑO-REPIZO, HÉCTOR GOICOECHEA, JULIO RABA, SOLEDAD CERUTTI

Salvador, Bahía

Workshop; VIII Workshop de Quimiometría - Simposio de Química Forense; 2017

Grupo de Pesquisa em Química e Quimiometría

Ochratoxin A (OTA) is a toxic compound, initiator, promoter of cancers and endemic diseases1. OTA contamination might occur when the ochratoxigenic fungi infect the cereal crops (mostly barley and wheat), during their cultivation and until post-harvest2. Thus, beer might contain OTA traces since brewing, cereals (malt) might be contaminated3. Therefore, some countries have established maximum allowable limits (MALs) to reduce OTA intake from beer (0.2 µg/L)4. For that reason, simple, effective and sensitive methods for OTA determination at low concentration levels are necessary5. Hence, a novel sample treatment methodology based on salting-out assisted liquid-liquid extraction (SALLE) was optimized using a multivariate design of experiments strategy and, then coupled to ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).