Microchip Electrophoresis Tools for the Analysis of Small Molecules

FEDERICO JOSE VICENTE GOMEZ; M. FERNANDA SILVA

Microfluidic Electrophoresis

Springer Nature

Año: 2019; p. 197 - 206

Microchip electrophoresis (ME) results from miniaturization of capillary electrophoresis (CE) to a microfabricated separation device. Both techniques have common characteristics, but in some aspects, the microfluidic separation device has unique features resulting from its planar miniaturized format. Here wedescribe the process to transfer of CE to ME and the benefits and drawbacks of the chip with respect to the capillary. A practical guide for method development on the microchip for small ionizable molecules such as phenolic compounds, amino acids, or alkaloids is also presented.