Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

ROMINA PAULA MONASTERIO; FONTANA, ARIEL R.; M. FERNANDA SILVA

ANALYTICAL METHODS

Royal Society of Chemistry

Lugar: Londres; Año: 2014 vol. 6 p. 8986 - 8995

1759-9660

Amethodology was developed usingmatrix solid-phase dispersion, togetherwith liquid chromatography with multiple wavelength detectors for the determination of 20 phenolic compounds in an olive oil samples. Under optimized conditions, the analytes were extracted using 0.5 g of olive oil, 1.0 g of Florisil as a sorbent and 1mL methanol?water (80 : 20 acidified with formic acid 0.5% (v/v)) as an eluting solvent. The proposed methodology provided detection and quantification limits of individual compounds in the ranges of 0.02? 0.75 and 0.08?2.50 mg kg
1, respectively. The RSDs resulting from the analysis of six replicates of 0.5 g of a sample pool containing 2.5 mg kg
1 phenolic compounds were ranged between 2.1% and 14.8%. Considering matrix-matched calibration as a quantification technique, the average recoveries ranged from 74.8% to 95.0% with relative standard deviations between 1.5% and 9.3%. The developed methodology was applied for the determination of phenolic compounds in nine olive oils produced in Argentina, identifying seventeen analytes at concentrations above the detectable levels