Phenolic characterization and antimicrobial activity of folk medicinal plant extracts for their applications in olive production

JOANA BOITEUX; SOTO VARGAS, CAROLINA; PIZZUOLO; PABLO; LUCERO; GABRIELA; M. FERNANDA SILVA

ELECTROPHORESIS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2014 vol. 35 p. 1709 - 1718

0173-0835

In this regard, treatment with plant extracts could be a valid strategy.The aims of the present study were a) to determine the polyphenol composition of extracts of Thymus vulgaris, Origanum vulgare, Matricaria recutita and Larrea divaricata by CZE, b) correlate the analytical composition of these extracts with the percentage of inhibition on the mycelial growth and c) determine the individual antimicrobial activity of the most active ingredients. A simple methodology was developed for the simultaneous determination of catechin, naringenin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in plant extracts by CZE.The extraction of phenolic compounds in extract was performed by a miniaturized solid phase extraction using a home-made minicolumn packed with suitable filtering material (C18, 50 mg) connected to a vacuum pump. The optimized analyses conditions were: 30 mM boric acid buffer, pH 9.50; capillary, 57 cm full length, 50 cm effective length, 75 μm ID, 375 μm OD; hydrodynamic injection at 30 mbar , 2 s; 25 kV constant voltage; 25 °C, detection by UV absorbance at 290 nm. Under the optimum conditions, the analytes were separated within 7 min. Sample results suggest that phenolic composition seems to have a great influence on the percentage of inhibition of P. palmivora, P. nicotianae and P. citrophthora. The greatest inhibitions of mycelial growth of the pathogens were observed for cinnamic acid and naringenin