INVESTIGADORES
SILVA Maria Fernanda
artículos
Título:
Simultaneous Determination of Dextromethorphan, Diphenhydramine and Phenylephrine in Expectorant and Decongestant Syrups by Capillary Electrophoresis
Autor/es:
M. ROXANA GÓMEZ; ROBERTO A. OLSINA; L. DANTE MARTÍNEZ; M. FERNANDA SILVA
Revista:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Editorial:
Elsevier
Referencias:
Año: 2002 vol. 30 p. 791 - 799
ISSN:
0731-7085
Resumen:
The separation of basic nitrogenous compounds commonly used as active ingredients in cold medicine formulations
by micellar electrokinetic capillary chromatography and capillary zone electrophoresis with direct absorptiometric
detection was investigated. The type and composition of the background electrolyte (BGE) were investigated with
respect to separation selectivity and BGE stability. BGE of 10 mM sodium dihydrogenphosphate?sodium tetraborate
buffer containing 10 mM SDS and 10% acetonitrile, pH 9.0 was found to be optimal. Dextromethorphan
hydrobhromide, diphenhydramine hydrochloride and phenylephrine hydrochloride were baseline-separated in less
than 11 min, giving separation efficiencies of up to 494,000 theoretical plates, reproducibility of corrected peaks areas
below 3% relative standard deviation and concentration detection limits from 2.5 to 5.5 g ml−1. Detection was
performed at 196 and 214 nm.g ml−1. Detection was
performed at 196 and 214 nm.