INVESTIGADORES
SEIJO Jose guillermo
artículos
Título:
BAC libraries from the ancestral diploid genomes of the allotetraploid cultivated peanut. BMC, Plant Biology 2008, 8:14. (USA).
Autor/es:
GHILMARHAES, P.; K. PROITE,; O. GARSMEUR; S.C.M. LEAL BERTIOLI; J.G. SEIJO; BERTIOLI, D.J; A. D¨HONT
Revista:
BMC PLANT BIOLOGY
Editorial:
BioMed Central The open access publisher
Referencias:
Año: 2008 vol. 8 p. 1 - 8
ISSN:
1471-2229
Resumen:
Background: Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an
AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck
was imposed at the species origin, via hybridisation of two wild species and spontaneous
chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the
crop´s low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis
species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore
attractive, both to simplify the construction of genetic and physical maps, and for the isolation and
characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A.
duranensis and A. ipaënsis with genome types AA and BB respectively.
Results: We constructed and characterized two large-insert libraries in Bacterial Artificial
Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and
BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and
average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones
containing genetically mapped legume anchor markers (single copy genes), and resistance gene
analogues.
Conclusion: These diploid BAC libraries are important tools for the isolation of wild alleles
conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB
genomes with each other and with other legume species, and will facilitate the construction of a
physical map.