PERSONAL DE APOYO
MARQUEZ Sebastian
artículos
Título:
Stard7 Knockdown Modulates ABCG2 expression, cell migration, proliferation and differentiation of Human Choricarcinoma JEG · Cells
Autor/es:
JESICA FLORES- MARTÍN; VIVIANA RENA; SEBASTIAN MARQUEZ; GRACIELA PANZETTA -DUTARI; SUSANA GENTI DE RAIMONDI
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2012 vol. 7 p. 1 - 11
ISSN:
1932-6203
Resumen:
Abstract
Background: StAR-related lipid transfer domain containing 7 (StarD7) is a member of the START-domain protein family
whose function still remains unclear. Our data from an explorative microarray assay performed with mRNAs from StarD7
siRNA-transfected JEG-3 cells indicated that ABCG2 (ATP-binding cassette sub-family G member 2) was one of the most
abundantly downregulated mRNAs.
Methodology/Principal Findings: Here, we have confirmed that knocking down StarD7 mRNA lead to a decrease in the
xenobiotic/lipid transporter ABCG2 at both the mRNA and protein levels (226.4% and 241%, p,0.05, at 48 h of culture,
respectively). Also a concomitant reduction in phospholipid synthesis, bromodeoxyuridine (BrdU) uptake and 3
H-thymidine
incorporation was detected. Wound healing and transwell assays revealed that JEG-3 cell migration was significantly
diminished (p,0.05). Conversely, biochemical differentiation markers such as human chorionic gonadotrophin b-subunit
(bhCG) protein synthesis and secretion as well as bhCG and syncytin-1 mRNAs were increased approximately 2-fold. In
addition, desmoplakin immunostaining suggested that there was a reduction of intercellular desmosomes between
adjacent JEG-3 cells after knocking down StarD7.
Conclusions/Significance: Altogether these findings provide evidence for a role of StarD7 in cell physiology indicating that
StarD7 modulates ABCG2 multidrug transporter level, cell migration, proliferation, and biochemical and morphological
differentiation marker expression in a human trophoblast cell mode