CONICET | Buscador de Institutos y Recursos Humanos

Summary: Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, amongthem, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizeswheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species,which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogensin cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina,and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole andto solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesiswas performed under controlled conditions in order to characterize root fungal colonization under both,optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, formingchlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogensources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Onlyamylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a ?true endophyte?;however further studies are necessary to elucidate its role in the association with wheat.

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