Surviving nebulization-induced stress: dexamethasone in pH-sensitive archaeosomes

MARIA JULIA ALTUBE, SOLANGE SELZER, MARCELO ALEXANDER DE FARIAS, RODRIGO V PORTUGAL, MARIA JOSE MORILLA AND EDER LILIA ROMERO

NANOMEDICINE

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2016

1743-5889

Aim: To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulization stress, pH-sensitive nanoliposomes (LpH) exhibiting archaeolipids, acting as ligands for scavenger receptors (pH-sensitive archaeosomes [ApH]), were prepared. Materials & methods: The anti-inflammatory effect of 0.18 mg DP/mg totallipid, 100?150 nm DP-containing ApH (dioleylphosphatidylethanolamine: Halorubrumtebenquichense total polar archaeolipids:cholesteryl hemisuccinate 4.2:2.8:3 w:w) was tested on different cell lines. Size and HPTS retention of ApH and conventional LpH (dioleylphosphatidylethanolamine:cholesteryl hemisuccinate 7:3 w:w) before and after nebulization were determined. Results & conclusion: DP-ApH suppressed IL-6 and TNF-α on phagocytic cells. Nebulized after 6-month storage, LpH increased size and completely lost its HPTS while ApH3 conserved size and polydispersity, fully retaining its original HPTS content.