INVESTIGADORES
SCHAIQUEVICH Paula Susana
congresos y reuniones científicas
Título:
Effect Of Melatonin On Prednisolone Eye Disposition In Cats
Autor/es:
DEL SOLE MJ; SCHAIQUEVICH P; ABA M; LANUSSE C; MORENO L
Reunión:
Congreso; Association for Research in Visual and Ophtahlmology; 2012
Resumen:
Purpose: To characterize prednisolone (PRED) ocular and systemic pharmacokinetics in cats after oral
administration and to study the effect of concomitant administration of melatonin (MEL) on PRED disposition.
Methods: Six (6) castrated young physically and ophthalmologically healthy male European Short Hair cats
were orally administrated with a single dose (10 mg) of PRED or a single dose of PRED (10 mg) and MEL (3
mg) in tablet. In anesthetized cats 2 mL of blood and 450 µL of aqueous humor (AH) were obtained from
preplaced cephalic antebrachial intravenous catheters and from one eye by direct puncture, respectively, at:
0.25, 0.5, 1, 1.5, 2, 3, 4 and 5 h after administration. Plasma and AH samples were assayed for PRED by
HPLC. A two-compartment model was used to simultaneously fit PRED plasma and aqueous concentration vs.
time data using ADAPT 5. The estimated pharmacokinetic (PK) parameters included the absorption rate
constant (ka), elimination rate constant from the central compartment (kc), intercompartment rate constants
between plasma and aqueous humor (kpa), apparent volume of distribution of the central compartment (Vc/F).
PK parameters were compared between groups (PRED vs. PRED+MEL) by means of Wilcoxon matched pairs
test (p<0.05).
Results: The model adequately fitted the data and the estimated median (interval) PK parameters are shown in
Table 1. No significant difference was observed in the PK parameters when comparing between groups of
treatments (p>0.05).
Conclusions: These results indicated that MEL does not modify PRED systemic or ocular disposition in cats
when both are administrated simultaneously. A possible synergic pharmacological effect may be account for
different mechanisms of action, but not for pharmacokinetic synergism and will be further studied.