INVESTIGADORES
SARNACKI Sebastian Hernan
congresos y reuniones científicas
Título:
Activation of iNKT cells prevents salmonella-enterocolitis and salmonella-induced reactive artrhitis by downregulating IL-17-producing γδt cells.
Autor/es:
NOTO LLANA MARIÁNGELES; KRUMMEL LUCÍA; MORALES ANDREA; SARNACKI SEBASTIÁN; GIACOMODONATO MÓNICA; BLANCO GUILLERMO; CERQUETTI MARÍA CRISTINA
Lugar:
CABA
Reunión:
Congreso; Reunión conjunta de Sociedades Biocientificas,; 2017
Institución organizadora:
Reunión conjunta de Sociedades Biocientificas,
Resumen:
Background: Reactive arthritis (ReA)is a sterile joint inflammation as a sequel to Salmonellagut infection. We have previously demonstrated that theseverity of joint lesions is directly related with the intestinal levelsof IL- 17 generated during S. Enteritidis infection.γδTlymphocytes are a possible source of IL-17. It has been suggested that γδT responses aremodulated by iNKT cells, therefore, here we analyze the involvement of γδT and iNKT cells on Salmonella-inducedReA. Methods: Adult female BALB/c mice received 3-4 x 10 3 colony forming unitsof S. Enteritidis by the gastrointestinal route.Studies were performed 1 and 5days after infection. Mesenteric lymph node γδT populationwas achieved by flow cytometry after enterocolitis onset.iNKT cell activation was studied using alpha-galactosylceramide (a- GalCer) and γδT activity was blocked with anti-γδTmonoclonal antibody. Then, histological tissueevaluation and mesenteric IL-17 expression by qPCR were assessed. Results: We found that during S.Enteritidis infection the total number of γδT cells is increased in mesentericlymph nodes. Infected mice treated with a-GalCer showed diminished intestinaland joint lesions concomitantly with a significantdecrease in mesenteric IL-17 expression. Animals suffering from enterocolitistreated with anti- γδT antibody presented the samephenomenon. In addition, mesenteric γδT population was decreased in number ina-GalCer t r e a t e d animals. Conclusions: Our results indicate thatactivation of iNKT cells renders protection against Salmonella ReA. This beneficial effect of a-GalCer treatment would be related to the decreasein IL-17 produced mainly by γδT cells.
