INVESTIGADORES
SARNACKI Sebastian Hernan
congresos y reuniones científicas
Título:
Intestinal inflammation and synovitis during non-lethal Salmonella enterocolitis in mice
Autor/es:
MARIANGELES NOTO LLANA; SEBASTIAN H SARNACKI; MONICA N GIACOMODONATO; ROBERTO L CACCURI; M CRISTINA CERQUETTI
Lugar:
Sapporo
Reunión:
Congreso; XIII International Congress of Bacteriology and Applied Microbiology; 2011
Institución organizadora:
International Union of Microbiological Societies (IUMS)
Resumen:
We investigated
inflammatory responses of intestine and knee joints in a mouse model for non-lethal
enterocolitis. BALB/c mice were
inoculated orally with 20 mg of streptomycin and 24 hs later they received 103
CFU of a virulent strain of Salmonella Enteritidis
by the same route. At different time post-infection animals were sacrificed and
samples were obtained. Salmonella was recovered from Peyer´s
patches up to day 4 and from spleen up to day 14. Bacteria were neither recovered from knee
joints nor from draining lymphnodes. In line with gut histological changes,
infected mice showed a significant increase (p < 0.05) in intestinal IL-17
and TNF-α, determined by qPCR. Twenty-one days post-infection animals presented
synovitis in the knee joints, suggesting that this non-lethal model of Salmonella enterocolitis is suitable for
studying reactive arthritis. We then
tested the effect of probiotics in preventing the joint sequelae. Thus, mice
were fed with commercially-available Lactobacillus
casei, ad libitum, for 7 days
before Salmonella infection. We found that Peyer´s patches and spleen of
mice pretreated with probiotics were less colonized with Salmonella, and for a shorter period. In addition, probiotic feeding previous to Salmonella infection resulted in a
significant decrease (p < 0.05) of gut inflammatory cytokines, compared to
infected animals not receiving Lactobacillus. TNF-α
levels decreased from 7.5 + 0.8 a.u. in infected mice to 1.4 + 0.5 a.u. in pretreated
animals. More dramatic, IL-17 intestinal
levels diminished from 24 + 4
a.u. in infected mice to 2.4 + 0.9 a.u. in pretreated
animals. Consequently, Salmonella-induced
synovitis was prevented in mice pretreated with probiotics. Altogether, our results showed that the non-lethal
model for enterocolitis might be useful not only for studying the pathogenesis
of Salmonella-induced synovitis but
for therapeutic and preventive approach.