Salmonella enterica serovar Enteritidis biofilm lifestyle induces lower pathogenicity and reducesinflammatory response in a murine model compared to planktonic bacteria

GIACOMODONATO M. N.; SARNACKI SH; MARÍA DEL ROSARIO AYA CASTAÑEDA; AILÍN N. GARÓFALOA; DIANA M. BETANCOURT; MARÍA C. CERQUETTI; MARIÁNGELES NOTO LLANA

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2021

0325-7541

Salmonella enterica serovar Enteritidis (S. Enteritidis) is the most frequent serovarinvolved in human salmonellosis. It has been demonstrated that about 80% of infections arerelated to biofilm formation. There is scant information about the pathogenicity of S. Enteri-tidis and its relationship to biofilm production. In this regard, this study aimed to investigatethe differential host response induced by S. Enteritidis biofilm and planktonic lifestyle. To thispurpose, biofilm and planktonic bacteria were inoculated to BALB/c mice and epithelial cellculture. Survival studies revealed that biofilm is less virulent than planktonic cells. Reducedsigns of intestinal inflammation and lower bacterial translocation were observed in animalsinoculated with Salmonella biofilm compared to the planktonic group. Results showed thatSalmonella biofilm was impaired for invasion of non-phagocytic cells and induces a lower inflam-matory response in vivo and in vitro compared to that of planktonic bacteria. Taken together,the outcome of Salmonella---host interaction varies depending on the bacterial lifestyle.© 2021 Asociaci´on Argentina de Microbiolog´ıa. Published by Elsevier Espa?na, S.L.U. This is anopen access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).