Echinococcus genomes: new information to apply to molecular epidemiology and echinococcosis control

KAMENETZKY, L.

Congreso; Congresso da Sociedade Brasileira de Medicina Tropical; 2019

The para<span class="keyword">sspan>ite Echinococcu<span class="keyword">sspan> &nb<span class="keyword">sspan>p;<span class="keyword">sspan>pp. i<span class="keyword">sspan>&nb<span class="keyword">sspan>p; the cau<span class="keyword">sspan>ative agent ofechinococco<span class="keyword">sspan>i<span class="keyword">sspan>, a worldwide chronic zoono<span class="keyword">sspan>i<span class="keyword">sspan> affecting human<span class="keyword">sspan> a<span class="keyword">sspan> well a<span class="keyword">sspan>dome<span class="keyword">sspan>tic and wild mammal<span class="keyword">sspan>. In South America <span class="keyword">sspan>ix <span class="keyword">sspan>pecie<span class="keyword">sspan>were found: E. granulo<span class="keyword">sspan>u<span class="keyword">sspan> <span class="keyword">sspan>en<span class="keyword">sspan>u <span class="keyword">sspan>tricto, E. canaden<span class="keyword">sspan>i<span class="keyword">sspan>, E. ortleppi, E.oligarthru<span class="keyword">sspan> and E . vogeli.&nb<span class="keyword">sspan>p; E.granulo<span class="keyword">sspan>u<span class="keyword">sspan> <span class="keyword">sspan>. <span class="keyword">sspan>.&nb<span class="keyword">sspan>p; account<span class="keyword">sspan> for mo<span class="keyword">sspan>t ofthe global burden of human and live<span class="keyword">sspan>tock ca<span class="keyword">sspan>e<span class="keyword">sspan>. Al<span class="keyword">sspan>o, E. canaden<span class="keyword">sspan>i<span class="keyword">sspan> play<span class="keyword">sspan> a <span class="keyword">sspan>ignificantrole in human cy<span class="keyword">sspan>tic echinococco<span class="keyword">sspan>i<span class="keyword">sspan>&nb<span class="keyword">sspan>p;particularly in Argentina, 36.1%&nb<span class="keyword">sspan>p; of the humanca<span class="keyword">sspan>e<span class="keyword">sspan> are cau<span class="keyword">sspan>ed by E. canaden<span class="keyword">sspan>i<span class="keyword">sspan>. We <span class="keyword">sspan>equenced &nb<span class="keyword">sspan>p;the completegenome of E. canaden<span class="keyword">sspan>i<span class="keyword">sspan> &nb<span class="keyword">sspan>p;and compared itwith the complete genome<span class="keyword">sspan> from E. multiloculari<span class="keyword">sspan>, E.granulo<span class="keyword">sspan>u<span class="keyword">sspan> (G1) and E. canaden<span class="keyword">sspan>i<span class="keyword">sspan> (G7). Genomicanaly<span class="keyword">sspan>e<span class="keyword">sspan> u<span class="keyword">sspan>ing whole genome data of three Echinococcu<span class="keyword">sspan><span class="keyword">sspan>pecie<span class="keyword">sspan> not only confirmed the <span class="keyword">sspan>tatu<span class="keyword">sspan> of E.canaden<span class="keyword">sspan>i<span class="keyword">sspan> (G7) a<span class="keyword">sspan> a <span class="keyword">sspan>eparate <span class="keyword">sspan>pecie<span class="keyword">sspan> but al<span class="keyword">sspan>o demon<span class="keyword">sspan>trated a highnucleotide <span class="keyword">sspan>equence<span class="keyword">sspan> divergence in relation to E. granulo<span class="keyword">sspan>u<span class="keyword">sspan> (G1). Comparative genomic<span class="keyword">sspan> revealed that there aremore <span class="keyword">sspan>ingle nucleotide polymorphi<span class="keyword">sspan>m<span class="keyword">sspan> (SNP<span class="keyword">sspan>) between E. canaden<span class="keyword">sspan>i<span class="keyword">sspan> (G7) and E.granulo<span class="keyword">sspan>u<span class="keyword">sspan> (G1) than between E.canaden<span class="keyword">sspan>i<span class="keyword">sspan> (G7) and E. multiloculari<span class="keyword">sspan>.Thi<span class="keyword">sspan> re<span class="keyword">sspan>ult wa<span class="keyword">sspan> unexpected <span class="keyword">sspan>ince E.canaden<span class="keyword">sspan>i<span class="keyword">sspan> (G7) and E. granulo<span class="keyword">sspan>u<span class="keyword">sspan>(G1) were con<span class="keyword">sspan>idered to belong to the <span class="keyword">sspan>pecie<span class="keyword">sspan> complex E. granulo<span class="keyword">sspan>u<span class="keyword">sspan> <span class="keyword">sspan>en<span class="keyword">sspan>u lato. At pre<span class="keyword">sspan>ent there i<span class="keyword">sspan> no unequivocaltaxonomic cla<span class="keyword">sspan><span class="keyword">sspan>ification of Echinococcu<span class="keyword">sspan><span class="keyword">sspan>pecie<span class="keyword">sspan> therefore additional ce<span class="keyword">sspan>tode genome<span class="keyword">sspan> <span class="keyword">sspan>uch a<span class="keyword">sspan> Echinococcu<span class="keyword">sspan> oligarthru<span class="keyword">sspan> are been <span class="keyword">sspan>equenced to re<span class="keyword">sspan>olve Echinococcu<span class="keyword">sspan>phylogeny. Additionally, relevant genomic <span class="keyword">sspan>tructure<span class="keyword">sspan> involved in gene expre<span class="keyword">sspan><span class="keyword">sspan>ionregulation were characterized among the three Echinococcu<span class="keyword">sspan> <span class="keyword">sspan>pecie<span class="keyword">sspan>.