First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers

CARNEVALE, S.; MALANDRINI, J.B; PANTANO, L.; SORIA C.; RODRUIGUES-SILVA, R.; MACHADO-SILVA, J.; VELÁSQUEZ, J.N.; KAMENETZKY, L.

VETERINARY PARASITOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017

0304-4017

Fasciola hepatica isa trematode showing genetic variation among isolates from different regionsof the world. The objective of this work was to characterize for the first timeF. hepatica isolates circulating indifferent regions of Argentina. Twenty-two adult flukes were collected fromnaturally infected bovine livers in different areas from Argentina and used forDNA extraction. We carried out PCR amplification and sequence analysis of theribosomal internal transcribed spacer 1 (ITS1), mitochondrial nicotinamideadenine dinucleotide dehydrogenase subunits 4 and 5 (nad4 and nad5) and mitochondrialcytochrome c oxidase subunit I (cox1)genes as genetic markers. Phylogenies were reconstructed using maximumparsimony algorithm. A total of 6 haplotypes werefound for cox1, 4 haplotypes for nad4 and 3 haplotypes for nad5. The sequenced ITS1 fragment wasidentical in all samples. The analyzedcox1 gene fragment is the most variable marker and is recommended forfuture analyses. No geographic association was found in the Argentinean samples