Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

COLUCCIO LESKOW, C.; KAMENETZKY, L.; DOMINGUEZ G.; DIAZ ZIRPOLO, J.; OBATA, T.; COSTA H.; MARTI, M.; TABOGA, O.; KEURENTJES J.; SULPICE, R.; ISHIHARA, H.; STITT, M.; FERNIE A. R.; CARRARI, F.

JOURNAL OF EXPERIMENTAL BOTANY

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2016

0022-0957

Improving carbon fixation in order to enhance crop yield i<span class="keyword">sspan> a major goal in plant <span class="keyword">sspan>cience<span class="keyword">sspan>. By quantitative trait locu<span class="keyword">sspan> (QTL) mapping, it ha<span class="keyword">sspan> been demon<span class="keyword">sspan>trated that a vacuolar inverta<span class="keyword">sspan>e (vac-Inv) play<span class="keyword">sspan> a key role in determining the radical length in Arabidop<span class="keyword">sspan>i<span class="keyword">sspan>. In thi<span class="keyword">sspan> model, variation in vac-Inv activity wa<span class="keyword">sspan> detected in a near i<span class="keyword">sspan>ogenic line (NIL) population derived from a cro<span class="keyword">sspan><span class="keyword">sspan> between two divergent acce<span class="keyword">sspan><span class="keyword">sspan>ion<span class="keyword">sspan>: Land<span class="keyword">sspan>berg erecta (Ler) and Cape Verde I<span class="keyword">sspan>land (CVI), with the CVI allele conferring both higher Inv activity and longer radicle<span class="keyword">sspan>. The aim of the current work i<span class="keyword">sspan> to under<span class="keyword">sspan>tand the mechani<span class="keyword">sspan>m(<span class="keyword">sspan>) underlying thi<span class="keyword">sspan> QTL by analyzing <span class="keyword">sspan>tructural and functional difference<span class="keyword">sspan> of vac-Inv from both acce<span class="keyword">sspan><span class="keyword">sspan>ion<span class="keyword">sspan>. Relative tran<span class="keyword">sspan>cript abundance analyzed by quantitative real-time PCR (qRT-PCR) <span class="keyword">sspan>howed <span class="keyword">sspan>imilar expre<span class="keyword">sspan><span class="keyword">sspan>ion pattern<span class="keyword">sspan> in both acce<span class="keyword">sspan><span class="keyword">sspan>ion<span class="keyword">sspan>; however, DNA <span class="keyword">sspan>equence analy<span class="keyword">sspan>e<span class="keyword">sspan> revealed <span class="keyword">sspan>everal polymorphi<span class="keyword">sspan>m<span class="keyword">sspan> that lead to change<span class="keyword">sspan> in the corre<span class="keyword">sspan>ponding protein <span class="keyword">sspan>equence. Moreover, activity a<span class="keyword">sspan><span class="keyword">sspan>ay<span class="keyword">sspan> revealed higher vac-Inv activity in genotype<span class="keyword">sspan> carrying the CVI allele than in tho<span class="keyword">sspan>e carrying the Ler allele. Analy<span class="keyword">sspan>e<span class="keyword">sspan> of purified recombinant protein<span class="keyword">sspan> <span class="keyword">sspan>howed a <span class="keyword">sspan>imilar K m for both allele<span class="keyword">sspan> and a <span class="keyword">sspan>lightly higher V max for that of Ler. Treatment of plant extract<span class="keyword">sspan> with foaming to relea<span class="keyword">sspan>e po<span class="keyword">sspan><span class="keyword">sspan>ible interacting Inv inhibitory protein(<span class="keyword">sspan>) led to a large increa<span class="keyword">sspan>e in activity for the Ler allele, but no change<span class="keyword">sspan> for genotype<span class="keyword">sspan> carrying the CVI allele. qRT-PCR analy<span class="keyword">sspan>e<span class="keyword">sspan> of two vac-Inv inhibitor<span class="keyword">sspan> in <span class="keyword">sspan>eedling<span class="keyword">sspan> from parental and NIL genotype<span class="keyword">sspan> revealed different expre<span class="keyword">sspan><span class="keyword">sspan>ion pattern<span class="keyword">sspan>. Taken together, the<span class="keyword">sspan>e re<span class="keyword">sspan>ult<span class="keyword">sspan> demon<span class="keyword">sspan>trate that the vac-Inv QTL affect<span class="keyword">sspan> root bioma<span class="keyword">sspan><span class="keyword">sspan> accumulation and al<span class="keyword">sspan>o carbon partitioning through a differential regulation of vac-Inv inhibitor<span class="keyword">sspan> at the mRNA level.