High-throughput characterization of Echinococcus spp. metacestode miRNomes

CUCHER, M.; MACCHIAROLI, N.; KAMENETZKY, L.; MALDONADO, L.; BREHM, K.; ROSENZVIT, M. C.

INTERNATIONAL JOURNAL FOR PARASITOLOGY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2015 vol. 45 p. 253 - 267

0020-7519

p { margin-bottom: 0.25cm; line-height: 120%; } Ab<span class="keyword">sspan>tract: Echinococco<span class="keyword">sspan>i<span class="keyword">sspan> i<span class="keyword">sspan> a worldwide zoono<span class="keyword">sspan>i<span class="keyword">sspan> of great public health concern, con<span class="keyword">sspan>idered a neglected di<span class="keyword">sspan>ea<span class="keyword">sspan>e by the WHO. The ce<span class="keyword">sspan>tode para<span class="keyword">sspan>ite<span class="keyword">sspan> Echinococcu<span class="keyword">sspan> granulo<span class="keyword">sspan>u<span class="keyword">sspan> <span class="keyword">sspan>en<span class="keyword">sspan>u lato (<span class="keyword">sspan>. l.) and Echinococcu<span class="keyword">sspan> multiloculari<span class="keyword">sspan> are the main aetiological agent<span class="keyword">sspan>. In the intermediate ho<span class="keyword">sspan>t, the<span class="keyword">sspan>e para<span class="keyword">sspan>ite<span class="keyword">sspan> di<span class="keyword">sspan>play particular developmental trait<span class="keyword">sspan> that lead to different pattern<span class="keyword">sspan> of di<span class="keyword">sspan>ea<span class="keyword">sspan>e progre<span class="keyword">sspan><span class="keyword">sspan>ion. In an attempt to under<span class="keyword">sspan>tand the cau<span class="keyword">sspan>e<span class="keyword">sspan> of the<span class="keyword">sspan>e difference<span class="keyword">sspan>, we focu<span class="keyword">sspan>ed on the analy<span class="keyword">sspan>i<span class="keyword">sspan> of microRNA<span class="keyword">sspan> (miRNA<span class="keyword">sspan>), <span class="keyword">sspan>mall non-coding regulatory RNA<span class="keyword">sspan> with major role<span class="keyword">sspan> in development of animal<span class="keyword">sspan> and plant<span class="keyword">sspan>. In thi<span class="keyword">sspan> work, we analyzed the <span class="keyword">sspan>mall RNA<span class="keyword">sspan> expre<span class="keyword">sspan><span class="keyword">sspan>ion pattern of the metace<span class="keyword">sspan>tode, the <span class="keyword">sspan>tage of <span class="keyword">sspan>anitary relevance, and provide a detailed de<span class="keyword">sspan>cription of Echinococcu<span class="keyword">sspan> miRNA<span class="keyword">sspan>. U<span class="keyword">sspan>ing high-throughput <span class="keyword">sspan>mall RNA <span class="keyword">sspan>equencing, we have carried out the fir<span class="keyword">sspan>t experimental identification of miRNA<span class="keyword">sspan> in E. multiloculari<span class="keyword">sspan> and have expanded the Echinococcu<span class="keyword">sspan> miRNA catalog to 38 miRNA gene<span class="keyword">sspan>, including one miRNA only pre<span class="keyword">sspan>ent in E. granulo<span class="keyword">sspan>u<span class="keyword">sspan> <span class="keyword">sspan>. l. Our finding<span class="keyword">sspan> <span class="keyword">sspan>how that although both <span class="keyword">sspan>pecie<span class="keyword">sspan> <span class="keyword">sspan>hare the top 5 highe<span class="keyword">sspan>t expre<span class="keyword">sspan><span class="keyword">sspan>ed miRNA<span class="keyword">sspan>, thirteen are differentially expre<span class="keyword">sspan><span class="keyword">sspan>ed which could be related to developmental difference<span class="keyword">sspan>. We al<span class="keyword">sspan>o provide evidence that uridylation i<span class="keyword">sspan> the main miRNA proce<span class="keyword">sspan><span class="keyword">sspan>ing mechani<span class="keyword">sspan>m in Echinococcu<span class="keyword">sspan> <span class="keyword">sspan>pp. Our re<span class="keyword">sspan>ult<span class="keyword">sspan> provide detailed information on Echinococcu<span class="keyword">sspan> miRNA<span class="keyword">sspan> which i<span class="keyword">sspan> the fir<span class="keyword">sspan>t <span class="keyword">sspan>tep to under<span class="keyword">sspan>tand their role on para<span class="keyword">sspan>ite biology and di<span class="keyword">sspan>ea<span class="keyword">sspan>e e<span class="keyword">sspan>tabli<span class="keyword">sspan>hment and/or progre<span class="keyword">sspan><span class="keyword">sspan>ion and their future potential u<span class="keyword">sspan>e a<span class="keyword">sspan> drug or diagno<span class="keyword">sspan>i<span class="keyword">sspan> target<span class="keyword">sspan>.