EXTRACTION OF LIPASE FROM ASPERGILLUS NIGER BY INSOLUBLE COMPLEX FORMATION WITH ANIONIC AND CATIONIC POLYELECTROLYTES
MARINI, ANALÍA; IMELIO, NATALIA; MARINI, SEBASTIAN; ROMANINI, DIANA; FARRUGGIA, BEATRIZ
Lugar: Amsterdam; Año: 2012 vol. 47 p. 2234 - 2234
The insoluble complex formation between lipase from Aspergillus niger and the electrically charged polymers, polyacrylic acid (PAA), poly-vinil sulfonate (PVS) and chitosan (CHI), was studied by using turbidimetric and enzymatic methods on a commercial lyophilized (Ly) and a ﬁltrate of solid culture medium (SCM). It could be shown that both electrostatic interactions as hydrophobic are involved in the formation of insoluble complexes. The kinetics of the complex formation were determined. Lipase enzymatic activity is maintained through time in the presence of polyelectrolytes. On the Ly the three polymers produced insoluble complex, with a stoichiometric ratio (polymer mass per mass of Ly from Aspergillus niger) of PAA/Ly: 0.035, PVS/Ly: 0.099 and CHI/Ly: 0.071mg/mg Ly. For the anionic polyelectrolytes, the PAA presents slightly better results than PVS to be used when the protein concentration is similar to the lyophilized. The ﬁltrate of the SCM has a total protein concentration much lower than commercial lyophilized. Working with CHI as cationic polymer a recovery of the activity in the re-dissolved precipitate higher than 80%, with puriﬁcation factors greater than 3 were achieved, both at 8 and 20◦C. Therefore, this methodology could be used as a ﬁrst step of puriﬁcation.