Upcycling of carrot discards into prebiotics (fructooligosaccharides) as high value food ingredients

GUERRA, LAUREANA; ROMANINI,DIANA; LOPEZ, SILVIA N; CASTELLI. MARÍA-VICTORIA; CLEMENTZ, ADRIANA L.

FOOD AND BIOPRODUCTS PROCESSING

INST CHEMICAL ENGINEERS

Año: 2023 vol. 132 p. 172 - 180

0960-3085

A great amount of carrots is discarded daily in the word because they do not meet thequality standards imposed by consumer market. The aim of this work was to evaluate thefeasibility of using carrot discards as substrate of solid-state fermentation to produce βfructofuranosidase by Aspergillus niger and its subsequent use to catalyze the synthesis offructooligosaccharides (prebiotics) using carrot juice. Results indicated that carrot bagassehas adequate nutrients for the growth of A. niger, but supplementation with a source ofnitrogen is required to obtain a high production of β-fructofuranosidase (90.82 U/mL).Maximum specific activity (251 U/mg) was obtained after 3 days of fermentation at 30 ºC,using an inoculum of 106 conidia/ mL. β-fructofuranosidase was successfully used tosynthesize fructooligosaccharides using carrot juice as substrate, raising the possibility forfuture development of a natural functional beverage.Tweetable abstract: A great amount of carrots is discarded daily. This paper evaluates theuse of carrot bagasse to produce β-fructofuranosidase and its subsequent application incarrot juice to produce a natural beverage with prebiotics