Acute exposition to Roundup Transorb® induces systemic oxidative stress and alterations in the expression of newly sequenced genes in silverside fish (Odontesthes humensis)

AMANDA W.S. MARTINS; TONY L.R. SILVEIRA; MARIANA H. REMIÃO; WILLIAM BORGES DOMINGUES; EDUARDO N. DELLAGOSTIN; ANTÔNIO S. VARELA JUNIOR; CARINE D. CORCINI; PATRÍCIA G. COSTA; ADALTO BIANCHINI; GUSTAVO M. SOMOZA; RICARDO B. ROBALDO; VINICIUS F. CAMPOS

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2021 vol. 28 p. 65127 - 65139

0944-1344

Roundup Transorb® (RDT) is a glyphosate-based herbicide used commonly in agricultural practices worldwide. The use of this herbicide is associated with negative effects on the aquatic ecosystem as well as changes in bioenergetic and detoxification pathways, oxidative stress, and cell damage in marine organisms. These effects might also occur at the transcriptional level; however, the expression of genes associated with oxidative stress has not been studied well. Odontesthes humensis is a native Brazilian aquatic species naturally distributed in the habitats affected by pesticides, including Roundup Transorb® (RDT). This study aimed to evaluate the toxic effects of short-term exposure to RDT on O. humensis. Moreover, the study aimed to sequence and characterize the genes related to oxidative stress and quantify their expression in the gills, hepatopancreas, kidneys, and brain of the fish by quantitative Reverse Transcription-polymerase chain reaction. The animals were exposed to two environmentally relevant concentrations of RDT (2.07 and 3.68 mg.L−1) for 24 h.Furthermore, lipid peroxidation, reactive oxygen species (ROS), DNA damage, and apoptosis in erythrocytes were quantified by flow cytometry. The results indicated modulation in the expression of the target genes in most tissues, mainly in the presence of the highest tested concentration of RDT. In erythrocytes, the levels of lipid peroxidation, ROS, and DNA damage were increased in the presence of both the concentrations of RDT, whereas cell apoptosis was increased in the group exposed to 3.68 mg.L−1 RDT. In conclusion, acute exposure to RDT caused oxidative stress in thefish, induced negative effects on cells, and modulated the expression of genes related to the enzymatic antioxidant system in O. humensis.