INVESTIGADORES
RODRIGUEZ andres Alberto
congresos y reuniones científicas
Título:
POLYAMINE CATABOLISM IN THE ELONGATION ZONE CONTRIBUTES TO SUSTAIN SOYBEAN HYPOCOTYLS GROWTH UNDER SALINE STRESS
Autor/es:
MARIA PAULA CAMPESTRE; SANTIAGO JAVIER MAIALE; CESAR BORDENAVE; ANDREA CECILIA ORIGONE; OSCAR ADOLFO RUIZ; ANDRES ALBERTO RODRIGUEZ
Lugar:
La Plata
Reunión:
Congreso; XXVIII Reunión Argentina de Fisiología Vegetal; 2010
Resumen:
Objetive:The
possible relationship between polyamine catabolism by
copper-containing amine oxidase and hypocotyl growth of salinized
soybean has been studied. Results: Salt treatment increased
spermine and mainly, cadaverine level in hypocotyls. Copper-containing
amine oxidase activity level was up to 77-fold higher that of the
polyamine oxidase. Copper-containing amine oxidase was active even in
salinity and showed highest affinity to cadaverine than putrescine. In vivo
determination of copper-containing amine oxidase activity in presence
of cadaverine, showed an increase with saline treatment modulated
negatively in treatment added with diaminoguanidine, a competitive
inhibitor. The same effects of cadaverine and diaminoguanidine were
observed in hypocotyl growth assays of under optimal or unfavourable
conditions. Additionally, plants treated with diaminoguanidine showed a
significative reduction of reactive oxygen species in the elongation
zone of hypocotyl detected by DCF fluorescence. Conclusions:
These results demostrated that copper-containing amine oxidase
activity produced hydrogen peroxide by polyamine catabolism in salinity
and this hydrogen peroxide production may be contributing to hypocotyl
growth partially under saline stress.