USE OF A POLYMERIC NANOPARTICLE AS AN ADJUVANT TO ACTIVATE MUCOSAL AND SYSTEMIC INNATE AND ADAPTIVE IMMUNITY

GASTÓN PASCUAL RIZZO; MAIA LINA ELIZAGARAY; CAMILA CHAVERO; DAIANA BIANCHI; EUGENIA APUZZO; SANTIAGO ESTEBAN HERRERA; MAXIMILIANO LUIS AGAZZI; GRISELDA MORENO; OMAR AZZARONI; GUILLERMO HORACIO DOCENA; PAOLA LORENA SMALDINI

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC . SAI . SAFIS; 2022

Sociedad Argentina de Inmunologia

Nanotechnology plays an important role in vaccine development. It offers the opportunity to design different functional nanoparticles (Np) based on different composition, size, shape and surface properties for biomedical applications. This work aimed to characterize Np as a safe vehicle and adjuvant to be further used in vaccines.Nanoparticles were characterized using human and murine antigen-presenting cells (APCs) and epithelial cells. Cell interaction was evaluated by fluorescence microscopy (internalization and localization), flow cytometry (activation-MHCII and CD86 expression) and ELISA (IL-1β secretion). Furthermore, Balb/c mice were intraperitoneally and intranasally administered with Np-OVA and the pharmacokinetic was monitored using Np-FITC. Finally, humoral and cellular immune responses (cell subsets and cytokines), and lung-resident memory T cells (Trm) were evaluated by ELISA and flow cytometry.We found that Np were internalized only by APC and cells became activated, showing a significant increased expression of CD86 and activation of the inflammasome with secretion of IL-1β. The IL-1β production was abrogated with different inflammasome inhibitors. In vivo experiments showed that Np protected OVA through the mucosa passage, and Np-OVA reached the critical organs to promote immune activation. We observed a significant induction of serum OVA-specific IgG, increased secretion of IFN-γ by splenocytes with a high frequency of CD8+- and CD4+-IFN-γ+ secreting cells. Remarkably, lung CD62L-CD69+ Trm cells (p