EFFECTS OF GLYPHOSATE (G) AND ROUNDUP (R) ON IMMATURE RAT SERTOLI CELL (SC) PROLIFERATION

GORGA, AGOSTINA; RINDONE GUSTAVO M; CENTOLA CECILIA LUCIA; PELLIZZARI ELIANA H; CAMBEROS MARÍA DEL CARMEN; RIERA M FERNANDA; GALARDO M NOEL; MERONI SILVINA B

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

A declining trend in human fertility has been described and exposure to xenobiotics, such as herbicides, emerges as a potential cause. We have shown that G and its commercial formulation R alter blood-testis barrier between neighboring SC, which would partly explain the decrease in reproductive function observed after herbicide exposure. This observation points the SC as a plausible target for G or R effects. In rats, SC proliferation occurs during fetal and postnatal periods up to 15 days of age. As each SC supports a limited number of germ cells, the number reached during proliferative periods will be decisive for spermatogenic capacity. Thus, disruption of any SC proliferative stage would compromise fertility. The aim of this work is to analyze whether exposure to G or R can alter postnatal SC proliferation. SC cultures from 8-day-old rats were treated with 100 ppm of G or R in the absence or presence of FSH, the main SC mitogen. Proliferation was evaluated by BrdU incorporation. It was observed that R, but not G, decreased FSH- stimulated SC proliferation (FSH: 19.8 ± 2.3; FSH + R: 9.7 ± 1.4*, X ± DS, *p