Effect of glucose deprivation on Sertoli cell lipid metabolism

RINDONE GUSTAVO M; GORGA AGOSTINA; REGUEIRA MARIANA; PELLIZZARI ELIANA H; CAMBEROS MARIA C; GALARDO, MARÍA N; MERONI SILVINA B; RIERA MARÍA F

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias.; 2017

Sociedad Argentina de Investigacion Clinica

Sertoli cells (SC) possess a unique metabolism based on its role as nutritional support for germ cells. SC actively metabolize glucose into lactate to provide energy to cells located in the adluminal compartment of seminiferous tubules and it has been demonstrated that SC can oxidize fatty acids (FA) and also store them as triacylglycerides (TAG) within lipid droplets (LD). We have previously demonstrated that SC are able to survive in culture for at least 48 h in the absence of glucose suggesting that, under this adverse condition, SC lipid metabolism would become essential for survival. However, no experiments regarding this matter were performed. The aim of the study was to investigate the effect of glucose deprivation on the regulation of lipid catabolism in SC. For this purpose, SC isolated from 20-day-old rats were cultured for 48 h in media containing different concentrations of glucose: 8 mM (concentration commonly used in the culture media of SC) and 0 mM. FA oxidation (measured as 3H2O production from [9,10-3H(N)]-palmitic acid and expressed as oxidized palmitate-Ox Palm), carnitine palmitoyltransferase 1A (CPT1A) expression (RT-qPCR), TAG levels (spectrophotometric method) and LD content (Red Oil O staining) were evaluated. Results are shown as means±S.D. of triplicate incubations in one representative experiment out of three. (*P