INVOLVEMENT OF GLUTAMINOLYSIS IN THE REGULATION OF SERTOLI CELL PROLIFERATION

CENTOLA CECILIA LUCIA; RINDONE GUSTAVO M; GORGA AGOSTINA; DASSO MARINA E; PELLIZZARI ELIANA H; CAMBEROS MARIA C; RIERA M FERNANDA; MERONI SILVINA B; GALARDO M NOEL

Congreso; Reunion Conjunta SAIC, SAI, AAEF, NANOMED.ra; 2021

SAIC

The final number of Sertoli cells (SC) reached during the proliferative periods determines spermproduction capacity in adulthood. FSH is the major SC mitogen and mTORC1/p70S6K pathway isinvolved in FSH-stimulated SC proliferation. On the other hand, glutaminase (GLS), whichconverts glutamine into glutamate, plays a vital role in up-regulating cell metabolism for cellgrowth, and even, the glutamine catabolism might be required for the fully activation ofmTORC1/p70S6K pathway. Previously, we have demonstrated that FSH increases the expressionof GLS isoform 2 in proliferating SC, however, the role of GLS activity in the regulation of SCproliferation remains unknown. The aim of this work was to analyze whether SC depends onglutaminolysis to proliferate. SC obtained from 8-day old rats were maintained in the absenceor presence of a pharmacological inhibitor of GLS -6-diazo-5-oxo-L-norleucine (DON) 500 μMunder basal conditions (B) or stimulated with FSH 100ng/ml. BrdU incorporation, c-Myctranscriptional activity by luciferase reporter assay after cell transfection and phosphorylatedp70S6K (P-p70S6K) levels by western blot were evaluated. Results are expressed as mean±SD ofthree independent experiments (Different letters indicate statistically significant differences,P