LEPTIN REGULATES THE EXPRESSION OF GENES RELATED TO LIPID STORAGE IN SERTOLI CELLS

DASSO MARINA E; RINDONE GUSTAVO M; GORGA AGOSTINA; CENTOLA CECILIA LUCIA; PELLIZZARI ELIANA H; CAMEROS MARIA C; GALARDO M NOEL; MERONI SILVINA B; RIERA M FERNANDA

Congreso; Reunion Conjunta SAIC, SAI, AAEF, NANOMED.ra; 2021

SAIC

Sertoli cells (SC) are necessary to provide an adequate environment for germ cell development.SC actively metabolize glucose but most of it is converted to lactate, an energy source for germcells. SC also oxidize fatty acids (FA) to sustain their energy status. SC have numerous lipiddroplets (LD), which are thought to be the site of storage of FA. In this context, we havedemonstrated that leptin (Lep), an adipokine that is present in the testis, increasestriacylglycerols (TAGs) and LD content in SC. Several proteins such as the FA transporterFAT/CD36, the enzymes involved in the synthesis of TAGs (glycerol-phosphate-acyl-transferases(GPATs) and diacylglycerol-acyl-transferase 1 (DGAT)), and the proteins associated with LDformation (PLINs) have a role in lipid storage. The aim of this study was to analyze the effects ofLep on the expression of proteins involved in FA storage. SC isolated from 20-day-old rats werecultured and maintained under basal conditions (B) or stimulated with Lep (100 ng/ml). mRNAlevels of FAT/CD36, GPAT1-4, DGAT1 and PLIN1-3 were analyzed by RT-qPCR. Results areexpressed as mean±SD, n=3, (* p