ASSESSMENT OF THE MECHANISMS UNDERLYING ACTIVIN A-INDUCED SERTOLI CELL PROLIFERATION

RINDONE GUSTAVO MARCELO; CENTOLA CECILIA LUCIA; GORGA AGOSTINA; DASSO MARINA E; PELLIZZARI ELIANA H; CAMEROS MARIA C; GALARDO MARÍA N; MERONI SILVINA B; RIERA M FERNANDA

Congreso; Reunion Conjunta SAIC, SAI, AAEF, NANOMED.ra; 2021

SAIC

Immature Sertoli cells (SC) proliferate to reach a steady population size that will ultimatelycorrelate with sperm output in adulthood. Activin A (ActA), a member of the TGF-β superfamily,acts as a paracrine factor on fetal and postnatal proliferation of SC. However, besides an increasein Cyclin D2 expression, little is known about the mechanisms that explain ActA-inducedproliferation of SC. The aim of this study was to assess the signaling pathways and geneexpression changes involved in ActA-induced proliferation of SC. SC were isolated from 8-dayold Sprague-Dawley rats and incubated under basal conditions (B) or stimulated with ActA (50ng/mL) during different periods of time. Results are expressed as X±SD, n=3 (*p