Extracellular and mycelium-bound lipase production by Penicillum corylophillum

. RODRÍGUEZ E., ROMERO C., KRIEGER N., CASTRO G.

Tafi del Valle. Tucuman

Jornada; XXII Jornadas Cientificas Asociación de Biología de Tucumán Tafi del Valle Tucumán; 2005

Sociedad de Biologìa de Tucuman

142. EXTRACELLULAR AND MYCELIUM-BOUND LIPASE PRODUCTION BY PENICILLIUM corylophillum Rodr.guez E, Romero C, Pera L, Krieger N, Castro G, Baigor. M. PROIMI Av. Belgrano y Pje. Caseros, 4000 Tucum.n. Tel: 4344888, E-mail: baigori@hotmail.com Introduction. Lipases (EC 3.1.1.3) are enzymes that catalyze the hydrolysis of triglycerides in the oil-water interface. Both extracellular and mycelium-bound lipases from fungi are important in industrial applications. In particular, the use of a naturally-bound lipase can be cost effective because the biomass can be used directly, thus eliminating isolation, purification and immobilization procedures. Objective. The aim of this work was the evaluation of both extracellular and mycelium-bound lipase activity production by a strain of Penicillium corylophillum. Materials and methods. P. corylophillum was grown at 30°C in MB medium with and without supplementation 2% olive oil. MB (g/l): sacarose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 0.2; CuSO4.5H2O 0.06; pH7. Lipase production was monitored using p-nitrophenylpalmitate as substrate. Both extracellular and mycelium-bound specific lipase activity was calculated. Results and conclusions. Specific activities obtaining in presence of olive oil were 1.5 U per mg of protein (supernatant) and 1.8 U per mg of dry weight (mycelium). This work was supported by grant CABBIO 2000 cod 012.