INVESTIGADORES
ROMERO Cintia Mariana
congresos y reuniones científicas
Título:
Determination of mycelium-bound lipase b-N-acetyl-D-glucosaminidase activity from Aspergillus niger MYA 135 in submerged process
Autor/es:
COLÌN V. ROMERO C, BAIGORÍ M, PERA L
Lugar:
Tafi del Valle. Tucuman
Reunión:
Jornada; XXII Jornadas Cientificas Asociación de Biología de Tucumán Tafi del Valle Tucumán; 2005
Institución organizadora:
Sociedad de Biologìa de Tucuman
Resumen:
DETERMINATION OF MYCELIUM-BOUND b-N-ACETYLD-
GLUCOSAMINIDASE ACTIVITY FROM ASPERGILLUS
niger MYA 135 IN SUBMERGED PROCESS
Col.n V, Romero C, Baigor. M, Pera L.
PROIMI Av. Belgrano y Pje. Caseros, 4000 Tucum.n. Tel: 4344888,
E-mail: lpera@hotmail.com
Introduction. Filamentous fungi grow by apical extension, localized
apical synthesis that creates a tubular hyphal morphology.
Besides linear tip extension, filamentous fungi branch to form new
intercalary growing tips. Wall lytic enzymes have an important role
in the process of apical growth. However, the events involved are
still not clearly understood. One of the wall lytic enzymes is the
activity b-N-acetyl-D-glucosaminidase (NAGase), that we use as
a relative marker of the wall lytic potential. Objective. Evaluation
of NAGase activity during fungal development in submerged process.
Materials and methods. Aspergillus niger (ATCC MYA 135)
was grown at 30°C in MB (g/l): sucrose 10; NH4NO3 2; KH2PO4 1;
MgSO4.7H2O 0.2; CuSO4.5H2O 0.06; pH5. NAGase activity was
determined using 0.01 g of wet mycelium and p-nitrophenyl-Nacetyl-
b-D-glucosaminide as substrate. Results and conclusions.
Both NAGase specific activity and branch frequency increase during
fungal growth. The maximum NAGase specific activity was
0.550U per mg of dry weight.