Irradiation and parasitism affect the ability of larval hemocytes of Anastrepha obliqua for phagocytosis and the production of reactive oxygen species

ITZIA GÓMEZ ALONSO; SHANTAL BALTIERRA URIBE; LUVIA SÁNCHEZ TORRES; MARIO CANCINO DIAZ; JUAN CANCINO DIAZ; SANDRA RODRIGUEZ MARTINEZ; SERGIO M. OVRUSKI; JORGE HENDRICHS; JORGE CANCINO

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2022

0739-4462

AbstractThe development of the parasitoid Doryctobracon crawfordi(Viereck) (Hymenoptera: Braconidae) in Anastrepha obliqua(McQuart) (Diptera: Tephritidae) larvae is unviable innature; however, if the host larva is irradiated at 160 Gy,the parasitoid develops and emerges successfully. Thissuggests that radiation affects the immune responses ofA. obliqua larvae, while the underlying mechanisms remainto be revealed. Using optical and electronic microscopieswe determined the number and type of hemocyte populationsfound inside the A. obliqua larvae, either nonirradiated,irradiated at 160 Gy, parasitized by D. crawfordi, orirradiated and parasitized. Based on flow cytometry, thecapacity to produce reactive oxygen species (ROS) wasdetermined by the 123‐dihydrorhodamine method in thosehemocyte cells. Five cell populations were found in thehemolymph of A. obliqua larvae, two of which (granulocytesand plasmatocytes) can phagocytize and produce ROS. Areduction in the number of cells, mainly of the phagocytictype, was observed, as well as the capacity of these cells toproduce ROS, when A. obliqua larvae were irradiated. Bothradiation and parasitization decreased the ROS productionand when A. obliqua larvae were irradiated followed byparasitization by D. crawfordi, the reduction of the ROSlevel was even greater. In contrast, a slight increase in thesize of these cells was observed in the hemolymph of theparasitized larvae compared to those in nonparasitizedlarvae. These results suggest that radiation significantlyaffects the phagocytic cells of A. obliqua and thus permitsthe development of the parasitoid D. crawfordi.