PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Comparison Of Dehalogenase Activity Between Pure And Mixed Cultures Of Actinomycetes
Autor/es:
FUENTES MS; SAEZ JM; BOURGUIGNON N; BENIMELI CS; AMOROSO MJ
Lugar:
Tucumán
Reunión:
Congreso; SAIB; 2009
Institución organizadora:
Sociedad de Química Biológica Argentina
Resumen:
Halogenated compounds are important environmental pollutants.
Lindane is a halogenated insecticide prohibited in countries. Its
bacterial degradation liberates chlorine by dehalogenases, playing
an important role in this process.
Lindane microbial degradation has been studied using pure and
mixed microbial cultures. Mixed cultures are suitable for
bioremediation because its biodiversity increase the catabolic
pathways available for contaminant biodegradation.
The aims of this work were to compare dehalogenase activities
between pure and mixed cultures of actinomycetes isolated from
pesticides contaminated soil, and its identification.
Streptomyces sp. M7, S. coelicolor A3 and four actinomycetes
isolates were cultivated alone in minimal medium (MM) with
lindane for acclimation. These strains, as pure and mixed cultures,
were cultivated in MM with lindane (1.66 mg L-1). Microbial cells
were used to obtain cell-free extracts for dechlorinase activity
assays. Enzyme activities ranged between 5.14 to 82.39 mmolCl-
/h/mg protein. The mixed culture A5-M7 showed the best activity
and it was higher than the sum of each activity of pure culture.
/h/mg protein. The mixed culture A5-M7 showed the best activity
and it was higher than the sum of each activity of pure culture.
isolates were cultivated alone in minimal medium (MM) with
lindane for acclimation. These strains, as pure and mixed cultures,
were cultivated in MM with lindane (1.66 mg L-1). Microbial cells
were used to obtain cell-free extracts for dechlorinase activity
assays. Enzyme activities ranged between 5.14 to 82.39 mmolCl-
/h/mg protein. The mixed culture A5-M7 showed the best activity
and it was higher than the sum of each activity of pure culture.
/h/mg protein. The mixed culture A5-M7 showed the best activity
and it was higher than the sum of each activity of pure culture.
These native streptomycetes present better
ability for lindane bioremediation in mixed cultures.